The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
Protein modification; protein ubiquitination.
Involvement in disease
Fanconi anemia complementation group L
Contains 1 RING-type zinc finger.
The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.
The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.