Overview

  • Product nameAnti-FANCL antibody
    See all FANCL primary antibodies
  • Description
    Goat polyclonal to FANCL
  • Specificityab105020 is expected to recognize both isoforms (NP_001108108.1; NP_060532.2).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-QNLKDVLEIDFP

    , corresponding to internal sequence amino acids 282-293 of Human FANCL (NP_060532.2).

  • Positive control
    • Human Bone Marrow lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab105020 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionUbiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseFanconi anemia complementation group L
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • DomainThe UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.
  • Post-translational
    modifications
    The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase FANCL antibody
    • FAAP43 antibody
    • Fancl antibody
    • FANCL_HUMAN antibody
    • Fanconi anemia complementation group L antibody
    • Fanconi anemia group L protein antibody
    • Fanconi anemia-associated polypeptide of 43 kDa antibody
    • FLJ10335 antibody
    • PHD finger protein 9 antibody
    • PHF9 antibody
    • POG antibody
    • Ubiquitin ligase protein FANCL antibody
    see all

Anti-FANCL antibody images

  • Anti-FANCL antibody (ab105020) at 1 µg/ml + Human Bone Marrow lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 43 kDa

References for Anti-FANCL antibody (ab105020)

ab105020 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"