The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
Protein modification; protein ubiquitination.
Involvement in disease
Fanconi anemia complementation group L
Contains 1 RING-type zinc finger.
The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.
The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling FANCL with ab94458 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm and nuclei of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - FANCL. Right - Merge.
Western blot - Anti-FANCL antibody (ab94458)
Anti-FANCL antibody (ab94458) at 1 µg/ml + Human fetal heart lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Gel concentration: 12%
Immunocytochemistry/ Immunofluorescence - Anti-FANCL antibody (ab94458)This image is courtesy of an anonymous Abreview
ab94458 staining FANCL in untreated HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 (0.5%) and blocked with 5% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/500 in PBS + 5% BSA + 0.05% Tween20) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.
Jamsai D et al. Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse. PLoS One8:e56955 (2013).
Read more (PubMed: 23451117) »
Jamsai D et al. Uncoupling of transcription and translation of Fanconi anemia (FANC) complex proteins during spermatogenesis. Spermatogenesis5:e979061 (0).
Read more (PubMed: 26413409) »