• Product nameAnti-FANCM antibody
    See all FANCM primary antibodies
  • Description
    Rabbit polyclonal to FANCM
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • Immunogen

    A region within N terminal amino acids 1-50 (MSGRQRTLFQ TWGSSISRSS GTPGCSSGTE RPQSPGSSKA PLPAAAEAQL) of Human FANCM (NP_065988.1).

  • Positive control
    • HeLa whole cell lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab95014 was affinity purified using an epitope specific to FANCM immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab95014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/2000 - 1/10000. Predicted molecular weight: 227 kDa.
IP Use at 5-15 µg/mg of lysate.


  • RelevanceFANCM is an ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. It binds to ssDNA but not to dsDNA.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • ATP-dependent RNA helicase FANCM antibody
    • FAAP250 antibody
    • Fanconi anemia group M protein antibody
    • fanconi anemia-associated polypeptide of 250 kDa antibody
    • KIAA1596 antibody
    • Protein Hef ortholog antibody
    see all

Anti-FANCM antibody images

  • All lanes : Anti-FANCM antibody (ab95014) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    developed using the ECL technique

    Predicted band size : 227 kDa

    Exposure time : 10 seconds
  • ab95014, at 0.4 µg/ml, detecting FANCM in HeLa whole cell lysate by WB following IP.
    Lane 1: ab95014 at 10 µg/mg of lysate
    Lane 2: IP with an antibody which recognizes a downstream epitope of FANCM
    Lane 3: control IgG.
    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 10 seconds
  • ab95014 staining FANCM in HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 2% Triton X-100 prior to blocking in 7% serum for 1 hour at room temperature. The primary antibody was diluted 1/100 in 0.1% Tween 20 in PBS and incubated with the sample for 17 hours at 4°C. The secondary antibody was Alexa Fluor® 555 conjugated Donkey anti-Rabbit polyclonal, diluted 1/1000.

    See Abreview

References for Anti-FANCM antibody (ab95014)

ab95014 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 2% TritonX10
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: RT°C

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Submitted Mar 09 2011