The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 52 kDa).
FunctionCatalyzes direct attachment of p-Tyr (Tyr) to tRNAPhe. Permits also, with a lower efficiency, the attachment of m-Tyr to tRNAPhe, thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins.
Sequence similaritiesBelongs to the class-II aminoacyl-tRNA synthetase family. Contains 1 FDX-ACB domain.