Overview

  • Product name
  • Description
    Rabbit polyclonal to FARSLA
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) from the N terminal region of FARSLA, conjugated to KLH.

  • Positive control
    • K562 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS)precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

Target

  • Relevance
    FARSLA (Phenylalanyl-tRNA synthetase alpha chain) is an aminoacyl-tRNA synthetase. These are a class of enzymes that charge tRNAs with their cognate amino acids. Cytoplasmic phenylalanine-tRNA synthetase is a heterodimer consisting of a catalytic alpha subunit, FARSLA, and a regulatory beta subunit, FARSLB. This protein has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase family shown to exhibit this type of regulated expression.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • CML 33 antibody
    • CML33 antibody
    • FARS antibody
    • FARSA antibody
    • FARSL antibody
    • FRSA antibody
    • PheHA antibody
    • Phenylalanine tRNA ligase 1 alpha cytoplasmic antibody
    • Phenylalanine tRNA ligase alpha chain antibody
    • Phenylalanine tRNA synthetase alpha subunit antibody
    • Phenylalanine tRNA synthetase like alpha subunit antibody
    • Phenylalanyl tRNA synthetase alpha chain antibody
    • Phenylalanyl tRNA synthetase alpha subunit antibody
    • Phenylalanyl tRNA synthetase like alpha subunit antibody
    • PheRS antibody
    see all

Images

  • Anti-FARSLA antibody (ab71437) at 1/60 dilution + K562 cell lysate at 35 µg

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ab71437 at 1/50 dilution staining FARSLA in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References

ab71437 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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