Overview

  • Product name
  • Description
    Rabbit polyclonal to Fas
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human Fas aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponding to Human Fas aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH). Database link: P25445
    Database link: P25445

  • Positive control
    • This antibody gave a positive signal in A431 cell line in IF/ICC.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 0.01-0.1µg for 106 cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • Tissue specificity
    Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • Involvement in disease
    Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • Sequence similarities
    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • Domain
    Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS 1A antibody
    • ALPS1A antibody
    • APO 1 antibody
    • Apo 1 antigen antibody
    • APO 1 cell surface antigen antibody
    • Apo-1 antigen antibody
    • APO1 antibody
    • Apo1 antigen antibody
    • APO1 cell surface antigen antibody
    • Apoptosis antigen 1 antibody
    • Apoptosis mediating surface antigen FAS antibody
    • Apoptosis-mediating surface antigen FAS antibody
    • APT 1 antibody
    • APT1 antibody
    • CD 95 antibody
    • CD 95 antigen antibody
    • CD95 antibody
    • CD95 antigen antibody
    • Delta Fas antibody
    • Delta Fas/APO 1/CD95 antibody
    • Delta Fas/APO1/CD95 antibody
    • Fas (TNF receptor superfamily, member 6) antibody
    • FAS 1 antibody
    • FAS 827dupA antibody
    • Fas AMA antibody
    • Fas antibody
    • FAS Antigen antibody
    • Fas cell surface death receptor antibody
    • FAS1 antibody
    • FASLG receptor antibody
    • FASTM antibody
    • sFAS antibody
    • Surface antigen APO1 antibody
    • TNF receptor superfamily, member 6 antibody
    • TNFRSF 6 antibody
    • TNFRSF6 antibody
    • TNR6_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 6 antibody
    see all

Images

  • Human peripheral blood lymphocytes stained with ab109913 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22ºC. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37ºC. For experimentation, cells were treated with 50% methanol (-20ºC) for 15 min at 4ºC. Cells were then incubated with the antibody (ab109913, 0.01μg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor&reg; 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rabbit IgG (polyclonal) (0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • ab109913 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109913 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab109913 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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