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Synthetic peptide (Mouse) derived from the extracellular domain, conjugated to KLH
Our Abpromise guarantee covers the use of ab82419 in the following tested applications.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 16339059|
|WB||1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
ab82419 staining CD95 in rat spleen tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in 4% paraformaldehyde, blocked with 10% donkey serum (ab7475) for 20 minutes at 24°C, then incubated with ab82419 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal (ab150073), used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).
ab82419 staining CD95 in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/50 in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) ab150073) was used as the secondary antibody.