Anti-Fas antibody [DX2] (Allophycocyanin) (ab25290)

Overview

  • Product nameAnti-Fas antibody [DX2] (Allophycocyanin)
    See all Fas primary antibodies
  • Description
    Mouse monoclonal [DX2] to Fas (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Specificityab25290 recognises CD95/Fas/Apo-1.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    ab25290 can be used for in vitro induction of apoptosis.

Properties

Applications

Our Abpromise guarantee covers the use of ab25290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. ab37391-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

In vitro induction of apoptosis.

Target

  • FunctionReceptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • Tissue specificityIsoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • Involvement in diseaseDefects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • Sequence similaritiesContains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • DomainContains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS 1A antibody
    • ALPS1A antibody
    • APO 1 antibody
    • Apo 1 antigen antibody
    • APO 1 cell surface antigen antibody
    • Apo-1 antigen antibody
    • APO1 antibody
    • Apo1 antigen antibody
    • APO1 cell surface antigen antibody
    • Apoptosis antigen 1 antibody
    • Apoptosis mediating surface antigen FAS antibody
    • Apoptosis-mediating surface antigen FAS antibody
    • APT 1 antibody
    • APT1 antibody
    • CD 95 antibody
    • CD 95 antigen antibody
    • CD95 antibody
    • CD95 antigen antibody
    • Delta Fas antibody
    • Delta Fas/APO 1/CD95 antibody
    • Delta Fas/APO1/CD95 antibody
    • Fas (TNF receptor superfamily, member 6) antibody
    • FAS 1 antibody
    • FAS 827dupA antibody
    • Fas AMA antibody
    • Fas antibody
    • FAS Antigen antibody
    • Fas cell surface death receptor antibody
    • FAS1 antibody
    • FASLG receptor antibody
    • FASTM antibody
    • sFAS antibody
    • Surface antigen APO1 antibody
    • TNF receptor superfamily, member 6 antibody
    • TNFRSF 6 antibody
    • TNFRSF6 antibody
    • TNR6_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 6 antibody
    see all

Anti-Fas antibody [DX2] (Allophycocyanin) images

References for Anti-Fas antibody [DX2] (Allophycocyanin) (ab25290)

ab25290 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: from spleen
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy alive cells
Username

Abcam user community

Verified customer

Submitted Jan 10 2013

Application Flow Cytometry
Sample Mouse Cell (splenocytes)
Specification splenocytes
Preparation Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy alive cells
Username

Abcam user community

Verified customer

Submitted Jan 02 2013

Application Flow Cytometry
Sample Cynomolgus Monkey Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive cells
Username

Abcam user community

Verified customer

Submitted Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive cells
Username

Abcam user community

Verified customer

Submitted Jan 02 2013

Thank you for contacting us. ab19456, ab11881, ab34359, ab25405 and ab25290 antibodies can be used in Biological studies which may include apoptosis. We unfortunately haven’t tested these antibodies in our own laboratory so unfortunately we will no...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"