Overview

  • Product nameFas (Apo-1) Human ELISA KitSee all CD95 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 91.24 82% - 105%
    Serum 84.46 78% - 102%
    Plasma 86.37 80% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Fas Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Fas coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Fas antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionReceptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • Tissue specificityIsoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • Involvement in diseaseDefects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • Sequence similaritiesContains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • DomainContains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • Cellular localizationSecreted and Cell membrane.
  • Target information above from: UniProt accession P25445 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • ALPS1A
    • APO 1
    • APO 1 cell surface antigen
    • Apo-1 antigen
    • APO1
    • Apo1 antigen
    • Apoptosis antigen 1
    • Apoptosis mediating surface antigen FAS
    • Apoptosis-mediating surface antigen FAS
    • APT1
    • CD95
    • CD95 antigen
    • Fas
    • Fas AMA
    • Fas antigen
    • FAS1
    • FASLG receptor
    • FASTM
    • TNF receptor superfamily member 6
    • TNFRSF6
    • TNR6_HUMAN
    • Tumor necrosis factor receptor superfamily member 6
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Fas (Apo-1) Human ELISA Kit images

  • Representative Standard Curve using ab100513.

  • Representative Standard Curve using ab100513.

Protocols

References for Fas (Apo-1) Human ELISA Kit (ab100513)

ab100513 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

Regarding the standard curve for ab100513 Fas Human ELISA Kit , I can confirm that the readings would be individual based on each individual experiment. A standard curve would need to be run each time.

...

Read More

Thank you for your enquiry.

Please find some useful information about the sample preparation below. It is important to note that the optimal sample preparation methods will need to be determined by the researcher.

In case follow-up ...

Read More

Thank you for contacting us and reporting the problems you have observed with the CD95 Human ELISA Kit (ab100513).

Fas is an alternative name for CD95, could you check for me if the 96 well plates were labeled with "Fas" or "Fa...

Read More

Thank you for your reply.

I can confirm that these kits will detect both soluble and membrane bound Fas and fas ligand.

In order to prevent membrane bound protein to interfere with your results, I suggest to spin down the saliva sam...

Read More

Thank you for your enquiry.

I have reviewed the details on the datasheets for these kits. For the Fas ligand kits:

ab45892 Fas Ligand Human ELISA Kit
ab100515 Fas Ligand Human ELISA Kit

Sensitivities are different:...

Read More

Thank you for your inquiry.

These ELISA kits are from two different sources but they are similar.

I recommend todetermine which is the best foryour needs from the datasheet and protocol information. Please take not of the different ...

Read More

Thank you for contacting us.

The best kit will depend on the amount of FAS (CD95) that you are expecting to detect in your samples. The kit ab100513 produces a standard curve that is linear for lower concentrations of FAS (CD95) than ab119571...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"