Synthetic peptide within Human Fas Ligand aa 1-60 (N terminal). The exact sequence is proprietary. Antibody will not interact with the soluble FasL corresponding to the C-term of the protein, the extracellular domain is in aa103-281. It will only react with the membrane form.
Prostate tissue slides.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
Involvement in disease
Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Belongs to the tumor necrosis factor family.
N-glycosylated. The soluble form derives from the membrane form by proteolytic processing.
Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
Fasl Fas ligand (TNF superfamily member 6) antibody
Generalized lymphoproliferative disease antibody
soluble form antibody
Tumor necrosis factor (ligand) superfamily member 6 antibody
Tumor necrosis factor ligand superfamily member 6 antibody
Immunohistochemistry (Frozen sections) - Anti-Fas Ligand antibody (ab15285)This image is courtesy of an anonymous Abreview
ab15285 staining Fas Ligand in mouse skeletal muscle blood vessel tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 2 hours at 25°C. The sample was incubated with primary antibody (1/100 in PBS-Tween 20) at 4°C for 12 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG (1/250) was used as secondary antibody.
ab15285 at 1/100 dilution staining Fas Ligand in human prostate tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
Immunocytochemistry/ Immunofluorescence - Anti-Fas Ligand antibody (ab15285)This image is courtesy of an anonymous Abreview
ab15285 staining Fas Ligand in Mouse macrophage RAAW264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody (1/1000).
Immunocytochemistry/ Immunofluorescence - Anti-Fas Ligand antibody (ab15285)This image is courtesy of an anonymous abreview.
ab15285 staining Fas Ligand in human 293ft cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 2 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence - Anti-Fas Ligand antibody (ab15285)Image courtesy of an anonymous Abreview
ab15285 staining Fas Ligand in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 12 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).
Liu G et al. Beclin-1-mediated Autophagy Protects Against Cadmium-activated Apoptosis via the Fas/FasL Pathway in Primary Rat Proximal Tubular Cell Culture. Sci Rep7:977 (2017).
Read more (PubMed: 28428545) »
Xiao J et al. Protective Effect of Met12, a Small Peptide Inhibitor of Fas, on the Retinal Pigment Epithelium and Photoreceptor After Sodium Iodate Injury. Invest Ophthalmol Vis Sci58:1801-1810 (2017).
Read more (PubMed: 28346613) »