Overview

  • Product nameAnti-Fas Ligand antibody
    See all Fas Ligand primary antibodies
  • Description
    Rabbit polyclonal to Fas Ligand
  • Tested applicationsSuitable for: IHC-Fr, ICC, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Fas Ligand aa 1-60 (N terminal). The exact sequence is proprietary. Antibody will not interact with the soluble FasL corresponding to the C-term of the protein, the extracellular domain is in aa103-281. It will only react with the membrane form.

  • Positive control
    • Prostate tissue slides.

Properties

Applications

Our Abpromise guarantee covers the use of ab15285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionCytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
  • Involvement in diseaseDefects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    N-glycosylated.
    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localizationCell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS1B antibody
    • Apoptosis (APO 1) antigen ligand 1 antibody
    • Apoptosis antigen ligand 1 antibody
    • Apoptosis antigen ligand antibody
    • APT1LG1 antibody
    • APTL antibody
    • CD178 antibody
    • CD178 antigen antibody
    • CD95 ligand antibody
    • CD95-L antibody
    • CD95L antibody
    • CD95L protein antibody
    • Fas antigen ligand antibody
    • Fas L antibody
    • Fas ligand (TNF superfamily member 6) antibody
    • Fas ligand antibody
    • FASL antibody
    • Fasl Fas ligand (TNF superfamily member 6) antibody
    • FASLG antibody
    • Generalized lymphoproliferative disease antibody
    • Gld antibody
    • soluble form antibody
    • TNFL6_HUMAN antibody
    • TNFSF6 antibody
    • Tumor necrosis factor (ligand) superfamily member 6 antibody
    • Tumor necrosis factor ligand superfamily member 6 antibody
    see all

Anti-Fas Ligand antibody images

  • ab15285 at 1/100 dilution staining Fas Ligand in human prostate tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • ab15285 staining Fas Ligand in mouse skeletal muscle blood vessel tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 2 hours at 25°C. The sample was incubated with primary antibody (1/100 in PBS-Tween 20) at 4°C for 12 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG (1/250) was used as secondary antibody.

    See Abreview

  • ab15285 staining Fas Ligand in human 293ft cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 2 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

  • ab15285 staining Fas Ligand in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 12 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

References for Anti-Fas Ligand antibody (ab15285)

This product has been referenced in:
  • Yang K  et al. Effect of PLCe gene silencing on inhibiting the cancerous transformation of ulcerative colitis. Exp Ther Med 12:422-426 (2016). WB ; Mouse . Read more (PubMed: 27347072) »
  • Schmid H  et al. Loss of inner retinal neurons after retinal ischemia in rats. Invest Ophthalmol Vis Sci 55:2777-87 (2014). Read more (PubMed: 24699380) »

See all 12 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Permeabilization No
Fixative Paraformaldehyde
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Verified customer

Submitted Apr 17 2014

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (4-12)
Sample Mouse Tissue lysate - whole (uterus)
Specification uterus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Varkha Agrawal

Verified customer

Submitted Nov 14 2013

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample Mouse Tissue sections (Placenta)
Specification Placenta
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (rat insulinoma cells)
Loading amount 20 µg
Specification rat insulinoma cells
Gel Running Conditions Reduced Denaturing (SDS boiling for 5 min)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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Submitted Nov 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Human Retina)
Specification Human Retina
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
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Submitted Sep 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (Kelly Neuroblastoma cell line)
Specification Kelly Neuroblastoma cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
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Submitted Sep 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Neuro2a)
Loading amount 6 µg
Specification Neuro2a
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Aug 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (B50 cell line)
Loading amount 6 µg
Specification B50 cell line
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Jul 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cultured Cells (33B)
Specification 33B
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
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Submitted Jul 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Neuro2a Cell Line)
Specification Neuro2a Cell Line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Jul 06 2012

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