Overview

  • Product nameAnti-Fas Ligand antibody [Alf1.2]See all Fas Ligand primary antibodies ...
  • Description
    Mouse monoclonal [Alf1.2] to Fas Ligand
  • Tested applicationsIP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant soluble active extracellular domain of Human FAS-Ligand.

  • EpitopeThis product specifically recognises the extracellular domain of human Fas ligand.
  • General notesIf slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab10511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Flow Cyt
  • Application notesFlow Cyt: Use at a concnetration of 1-2 µg/test. Determined by flow cytometry using cultured KFL9 cells (human chronic myelogenous leukemia cell line K562, transfected with human Fas ligand). When assayed by flow cytometric analysis, using the antibody at working concentration to stain 1 x 106 to 2 x 106 cells/ 0.1ml/ test, fluorescence intensity is observed similar to that obtained with saturating antibody levels. The percentage population positive is also at the maximum percentage positive, using saturating antibody levels.
    IP: Use at an assay dependent dilution. Observed to bind an unknown 55 kDa protein (antigen = 33 kDa) during immunoprecipitation.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
    • Involvement in diseaseDefects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
    • Sequence similaritiesBelongs to the tumor necrosis factor family.
    • Post-translational
      modifications
      N-glycosylated.
      The soluble form derives from the membrane form by proteolytic processing.
    • Cellular localizationCell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALPS1B antibody
      • APO1L antibody
      • Apoptosis (APO 1) antigen ligand 1 antibody
      • Apoptosis antigen ligand 1 antibody
      • Apoptosis antigen ligand antibody
      • APT1LG1 antibody
      • APTL antibody
      • CD178 antibody
      • CD178 antigen antibody
      • CD95 ligand antibody
      • CD95-L antibody
      • CD95L antibody
      • CD95L protein antibody
      • FAS antigen ligand antibody
      • Fas L antibody
      • Fas ligand (TNF superfamily member 6) antibody
      • Fas ligand antibody
      • FASL antibody
      • Fasl Fas ligand (TNF superfamily member 6) antibody
      • FASLG antibody
      • Generalized lymphoproliferative disease antibody
      • Gld antibody
      • soluble form antibody
      • TNF superfamily member 6 antibody
      • TNFL6_HUMAN antibody
      • TNFSF6 antibody
      • Tumor necrosis factor (ligand) superfamily member 6 antibody
      • Tumor necrosis factor ligand superfamily member 6 antibody
      see all

    References for Anti-Fas Ligand antibody [Alf1.2] (ab10511)

    This product has been referenced in:
    • Drukker M  et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). Human . Read more (PubMed: 22634564) »
    • Smith D  et al. Technical note: Aberrant detection of cell surface Fas ligand with anti-peptide antibodies. J Immunol 160:4159-60 (1998). Read more (PubMed: 9574514) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"