The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use at a concnetration of 1-2 µg/test. Determined by flow cytometry using cultured KFL9 cells (human chronic myelogenous leukemia cell line K562, transfected with human Fas ligand). When assayed by flow cytometric analysis, using the antibody at working concentration to stain 1 x 106 to 2 x 106 cells/ 0.1ml/ test, fluorescence intensity is observed similar to that obtained with saturating antibody levels. The percentage population positive is also at the maximum percentage positive, using saturating antibody levels.
IP: Use at an assay dependent dilution. Observed to bind an unknown 55 kDa protein (antigen = 33 kDa) during immunoprecipitation.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
Involvement in diseaseDefects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Sequence similaritiesBelongs to the tumor necrosis factor family.
Post-translational modificationsN-glycosylated. The soluble form derives from the membrane form by proteolytic processing.
Cellular localizationCell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
Fasl Fas ligand (TNF superfamily member 6) antibody
Generalized lymphoproliferative disease antibody
soluble form antibody
Tumor necrosis factor (ligand) superfamily member 6 antibody
Tumor necrosis factor ligand superfamily member 6 antibody
References for Anti-Fas Ligand antibody [Alf1.2] (ab10511)
This product has been referenced in:
Drukker M et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol30:531-42 (2012).
Read more (PubMed: 22634564) »
Smith D et al. Technical note: Aberrant detection of cell surface Fas ligand with anti-peptide antibodies. J Immunol160:4159-60 (1998).
Read more (PubMed: 9574514) »