Anti-Fas Ligand antibody [SB93a] (FITC) (ab87023)

Overview

  • Product name
    Anti-Fas Ligand antibody [SB93a] (FITC)
    See all Fas Ligand primary antibodies
  • Description
    Mouse monoclonal [SB93a] to Fas Ligand (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Reacts with native and recombinant Human Fas Ligand.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human protein

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab87023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µl for 106 cells. ab91368-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
  • Involvement in disease
    Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    N-glycosylated.
    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localization
    Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS1B antibody
    • Apoptosis (APO 1) antigen ligand 1 antibody
    • Apoptosis antigen ligand 1 antibody
    • Apoptosis antigen ligand antibody
    • APT1LG1 antibody
    • APTL antibody
    • CD178 antibody
    • CD178 antigen antibody
    • CD95 ligand antibody
    • CD95-L antibody
    • CD95L antibody
    • CD95L protein antibody
    • Fas antigen ligand antibody
    • Fas L antibody
    • Fas ligand (TNF superfamily member 6) antibody
    • Fas ligand antibody
    • FASL antibody
    • Fasl Fas ligand (TNF superfamily member 6) antibody
    • FASLG antibody
    • Generalized lymphoproliferative disease antibody
    • Gld antibody
    • soluble form antibody
    • TNFL6_HUMAN antibody
    • TNFSF6 antibody
    • Tumor necrosis factor (ligand) superfamily member 6 antibody
    • Tumor necrosis factor ligand superfamily member 6 antibody
    see all

Images

  • Flow Cytometry analysis of 1,10-Phenanthroline-stimulated human peripheral blood lymphocytes labeling Fas Ligand with ab87023 at 1 μg/106 cells (purple). Grey - Isotype Control, Mouse IgG2b-FITC.

  • ab87023 at 1µg/ml, staining Human PBMCs stimulated with (red line) PMA/ionomycin, then blocked with an MMP7 inhibitor (dotted line corresponds to unstimulated cells)

References

This product has been referenced in:
  • Bhogal RH  et al. Activation of CD40 with Platelet Derived CD154 Promotes Reactive Oxygen Species Dependent Death of Human Hepatocytes during Hypoxia and Reoxygenation. PLoS One 7:e30867 (2012). Flow Cyt ; Human . Read more (PubMed: 22295117) »
  • Carney EF  et al. Classical ataxia telangiectasia patients have a congenitally aged immune system with high expression of CD95. J Immunol 189:261-8 (2012). Human . Read more (PubMed: 22649200) »

See all 2 Publications for this product

Customer reviews and Q&As


For stimulation, 1 µg/ml of PMA (Sigma, P1585) and 5 µg/ml of Ionomycin (Sigma, I0634) were added to cells and incubated for 2 hours. For the last 30 minutes of this 2 hour incubation, 0.1mM of 10PM (I, 10- Phenanthroline Monohydrate) ...

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The final concentration of 10PM should be around 0.1mM. The 1µg/mL of PMA and 5µg/mL of Ionomycin are the final concentrations in the cell supsension. Also, when adding 10PM, add it in the last thirty minutes of the 2 hour stimulation.

While we believe that this product will work with soluble Fas Ligand, we have not tested this antibody to confirm.

The protocol for the positive control for flow cytometry is given below:

1 µg/ml of PMA (Sigma, P1585) and 5 &mic...

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Thank you for your email. Please find below the answers to your questions : 1. I would like to know how many cells you used for the stimulation, Answer : Typically we use approximately 0.5 x 106 cells per tube 2. the concentration of PM...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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