Anti-Fascin 2 antibody (ab78599)
- Datasheet
- Specific References (1)
- Protocols
Overview
-
Product nameAnti-Fascin 2 antibody
See all Fascin 2 primary antibodies -
DescriptionGoat polyclonal to Fascin 2
-
Host speciesGoat
-
Specificityab78599 is expected to recognize both reported isoforms (NP_001070650.1 and NP_036550.1) of Fascin 2.
-
Tested applicationsSuitable for: ICC/IF, WBmore details
-
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Dog, Human
-
Immunogen
Synthetic peptide:
CHHRGSNQLDTNR
, corresponding to internal sequence amino acids of Human Fascin 2 (NP_001070650.1 and NP_036550.1) -
Positive control
- Mouch cochlea.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesab78599 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab78599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Preliminary experiments gave bands at approx 75kDa and 22kDa in Mouse Eye lysates after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). |
Target
-
FunctionActs as an actin bundling protein. May play a pivotal role in photoreceptor cell-specific events, such as disk morphogenesis.
-
Tissue specificityLocalized specifically in the outer and inner segments of the photoreceptor cells in the retina.
-
Involvement in diseaseDefects in FSCN2 are the cause of retinitis pigmentosa type 30 (RP30) [MIM:607921]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP30 inheritance is autosomal dominant.
-
Sequence similaritiesBelongs to the fascin family.
-
Cellular localizationCytoplasm > cytoskeleton. Cell projection > stereocilium.
- Information by UniProt
-
Database links
- Entrez Gene: 337926 Cow
- Entrez Gene: 483362 Dog
- Entrez Gene: 25794 Human
- Entrez Gene: 238021 Mouse
- Entrez Gene: 303741 Rat
- Omim: 607643 Human
- SwissProt: O18728 Cow
- SwissProt: O14926 Human
see all -
Alternative names
- Fascin homolog 2, actin bundling protein, retinal Strongylocentrotus purpuratus antibody
- Fascin Strongylocentrotus purpuratus homolog 2 actin bundling protein, retinal antibody
- Fascin-2 antibody
see all
References
This product has been referenced in:
- Sui X et al. p53 controls colorectal cancer cell invasion by inhibiting the NF-?B-mediated activation of Fascin. Oncotarget 6:22869-79 (2015). Read more (PubMed: 26362504) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"