Overview

  • Product name
  • Description
    Rabbit polyclonal to Fascin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human Fascin aa 89-365. The exact sequence is proprietary.
    Database link: Q16658

  • Positive control
    • Mouse ovary tissue, HCT116 cells, HeLa whole cell lysate/extract

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.0
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 79% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the fascin family.
  • Domain
    Composed of four beta-trefoil domains.
  • Post-translational
    modifications
    Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium.
  • Information by UniProt
  • Database links
  • Alternative names
    • 55 kDa actin bundling protein antibody
    • 55 kDa actin-bundling protein antibody
    • Actin bundling protein antibody
    • actin bundling protein, 55-KD antibody
    • FAN 1 antibody
    • FAN1 antibody
    • Fascin 1 antibody
    • Fascin actin bundling protein 1 antibody
    • Fascin antibody
    • Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) antibody
    • Fascin homolog 1 antibody
    • Fascin, sea urchin, homolog of, 1 antibody
    • Fascin1 antibody
    • FLJ38511 antibody
    • FSCN 1 antibody
    • FSCN1 antibody
    • FSCN1_HUMAN antibody
    • HSN antibody
    • p55 antibody
    • Singed (Drosophila) like (sea urchin fascin homolog like) antibody
    • Singed drosophila homolog like antibody
    • Singed like (fascin homolog sea urchin) (Drosophila) antibody
    • Singed like (fascin homolog sea urchin) antibody
    • Singed like protein antibody
    • Singed, drosophila, homolog of antibody
    • Singed-like protein antibody
    • SNL antibody
    • Strongylocentrotus purpuratus antibody
    see all

Images

  • Anti-Fascin antibody (ab183891) at 1/1000 dilution + HeLa whole cell lysate/extract at 30 µg

    Predicted band size: 54 kDa

  • Immunofluorescence analysis of HCT116 cells fixed in 100% methanol for 5 minutes, labeling Fascin using ab183891 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.

  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue, labeling Fascin using ab183891 at a 1/500 dilution.

References

This product has been referenced in:
  • Hao YJ  et al. Role of RNA-interference-induced zinc finger protein 139 suppression in gastric cancer cell sensitivity to chemotherapeutic agents. Oncol Lett 10:1333-1338 (2015). WB . Read more (PubMed: 26622672) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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