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DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.
Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
Fast DNA modification Kit (ab117127) allows DNA denaturation and bisulfite conversion to be processed at the same time so the complete procedure can be performed in only 30 minutes. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil.
Traditional methods involve a separate denaturation step followed by a subsequent sodium bisulfite DNA conversion step - but with ab117127, DNA denaturation status is concurrently sustained throughout the entire bisulfite DNA conversion process. ab117127 is suitable for MS-PCR, real time MS-PCR, bisulfite sequencing, pyrosequencing and methylation microarray.
Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.
|Capture Solution||1 x 15ml|
|Conversion Enhancer||1 x 5 vials|
|Conversion Mix Solution||1 x 6ml|
|Denaturation Enhancer||1 x 600µl|
|Desulphonation Solution||1 x 60µl|
|Elution Solution||1 x 1ml|
|F-Collection Tube||1 pack|
|F-Spin Column||1 pack|
Our Abpromise guarantee covers the use of ab117127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration. Minimum DNA amount: 200pg-1µg/ reaction. Optimal DNA amount: 50-200ng/ reaction|
ab117127 has not yet been referenced specifically in any publications.