Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)

Overview

  • Product name
    Anti-Fast Myosin Skeletal Heavy chain antibody
    See all Fast Myosin Skeletal Heavy chain primary antibodies
  • Description
    Rabbit polyclonal to Fast Myosin Skeletal Heavy chain
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Sheep, Guinea pig, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Fast Myosin Skeletal Heavy chain.

  • Positive control
    • This antibody gave a positive signal in the following lysates: Skeletal Muscle (Human) Tissue Lysate; Skeletal Muscle (Mouse) Tissue Lysate; Skeletal Muscle (Rat) Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 23515448
WB Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function
    Muscle contraction.
  • Sequence similarities
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization
    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • adult 1 antibody
    • MYH1 antibody
    • MYH1_HUMAN antibody
    • MyHC-2x antibody
    • MyHC-IIx/d antibody
    • Myosin heavy chain 1 antibody
    • Myosin heavy chain 2x antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIx/d antibody
    • Myosin-1 antibody
    • skeletal muscle antibody
    see all

Images

  • IHC image of Fast Myosin Skeletal Heavy chain staining in Mouse skeletal muscle FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • All lanes : Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 223 kDa
    Observed band size : 223 kDa


    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab91506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Immunohistochemical analysis of sheep muscle tissue frozen section, labeling Fast Myosin Skeletal Heavy Chain with ab91506. Samples were fixed in methanol, blocking was with 3% BSA for 30 minutes at 25°C. Samples were incubated with ab91506 diluted 1/200 for 16 hours at 4°C. 

    See Abreview

References

This product has been referenced in:
  • Wan H  et al. Transfer of ß-hydroxy-ß-methylbutyrate from sows to their offspring and its impact on muscle fiber type transformation and performance in pigs. J Anim Sci Biotechnol 8:2 (2017). Read more (PubMed: 28074127) »
  • Stuart CA  et al. Myosin content of individual human muscle fibers isolated by laser capture microdissection. Am J Physiol Cell Physiol 310:C381-9 (2016). Read more (PubMed: 26676053) »

See all 8 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Tissue lysate - other (muscle)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
10 µg
Specification
muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Osnat Ophir

Verified customer

Submitted Oct 02 2017

Application
Immunocytochemistry
Sample
Rat Cell (Muscle Tissue)
Permeabilization
Yes - TritonX 0,1%
Specification
Muscle Tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Nov 02 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Sheep Tissue sections (muscle)
Permeabilization
No
Specification
muscle
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Oct 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up