Anti-Fast Myosin Skeletal Heavy chain antibody [LM5] (ab97714)

Overview

  • Product nameAnti-Fast Myosin Skeletal Heavy chain antibody [LM5]
    See all Fast Myosin Skeletal Heavy chain primary antibodies
  • Description
    Mouse monoclonal [LM5] to Fast Myosin Skeletal Heavy chain
  • SpecificityThis antibody recognises all fast myosin heavy chains (embryonic, neonatal, adult) in chicks but reacts with neonatal and adult rat/mouse myosin heavy chains showing no reaction with the embryonic/fetal isoform.
  • Tested applicationsSuitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Purified Chicken Pectorial Muscle.

  • Positive control
    • Rodent or Avian Skeletal Muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab97714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 223 kDa.
IHC-Fr Use at an assay dependent dilution.

Target

  • FunctionMuscle contraction.
  • Sequence similaritiesContains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • adult 1 antibody
    • MYH1 antibody
    • MYH1_HUMAN antibody
    • MyHC-2x antibody
    • MyHC-IIx/d antibody
    • Myosin heavy chain 1 antibody
    • Myosin heavy chain 2x antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIx/d antibody
    • Myosin-1 antibody
    • skeletal muscle antibody
    see all

References for Anti-Fast Myosin Skeletal Heavy chain antibody [LM5] (ab97714)

This product has been referenced in:
  • Williams K & Dhoot GK Heterogeneity and distribution of fast myosin heavy chains in some adult vertebrate skeletal muscles. Histochemistry 97:361-70 (1992). Read more (PubMed: 1618651) »
  • Dhoot GK Changes in the distribution of slow skeletal myosin heavy chain SM1 in developing avian muscle fibres. J Muscle Res Cell Motil 9:120-31 (1988). Read more (PubMed: 2458381) »

See all 2 Publications for this product

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