Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)


  • Product nameAnti-Fast Myosin Skeletal Heavy chain antibody [MY-32]
    See all Fast Myosin Skeletal Heavy chain primary antibodies
  • Description
    Mouse monoclonal [MY-32] to Fast Myosin Skeletal Heavy chain
  • Specificityab51263 is specific for the myosin heavy chain from skeletal muscle extracts or purified myosin preparations. In particular, it stains the fast twitch (type II) isomyosin molecules. It does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Human
  • Immunogen

    Full length native protein (purified) corresponding to Rabbit Fast Myosin Skeletal Heavy chain. Purified rabbit muscle myosin.

  • Positive control
    • Human skeletal muscle
  • General notes

    May be used for detecting cross striated muscle differentiation in tumors.



Our Abpromise guarantee covers the use of ab51263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 223 kDa.
IHC-P 1/400.
ICC/IF Use at an assay dependent concentration.


  • FunctionMuscle contraction.
  • Sequence similaritiesContains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • adult 1 antibody
    • MYH1 antibody
    • MYH1_HUMAN antibody
    • MyHC-2x antibody
    • MyHC-IIx/d antibody
    • Myosin heavy chain 1 antibody
    • Myosin heavy chain 2x antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIx/d antibody
    • Myosin-1 antibody
    • skeletal muscle antibody
    see all

Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] images

  • ab51263 staining Fast Myosin Skeletal Heavy chain in murine abdominal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263)

This product has been referenced in:
  • Lee I  et al. Deletion of heart-type cytochrome c oxidase subunit 7a1 impairs skeletal muscle angiogenesis and oxidative phosphorylation. J Physiol 590:5231-43 (2012). Read more (PubMed: 22869013) »
  • Jiang Y  et al. Variability in the muscle composition of rat esophagus and neural pathway of lower esophageal sphincter relaxation. Am J Physiol Gastrointest Liver Physiol 301:G1014-9 (2011). IHC-P ; Rat . Read more (PubMed: 21885684) »

See all 5 Publications for this product

Product Wall

I can confirm that ab51263 Fast Myosin Skeletal Heavy chain antibody [MY-32] was produced from spleen of mouse immunised with rabbit muscle myosin. I am sorry that regrettably no other specification as to its epitope identity has been assessed. Neverth...

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r these details as this enables us to closely monitor the quality of our products

We would be pleased to arrange a replacement product free of charge in order number#

Thank you for your help and cooperation. Please do not hesitate...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

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Apologies for the delay, I was waiting for further information.

Our products team has confirmed that these products have the correct alternative names listed. As stated on the datasheets they will specifically stain either the fast or slow f...

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According to our records, ab51263 was proving difficult to use in WB and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the di...

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Thank you for contacting us. We have following products available in catalogue that are best match as per your request. ab11083 ab51263 ab104128 I hope this information is helpful to you. Please do not hesitate to contact us if you need any...

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I am sorry that I couldn't help the customer on this occasion.

I have checked the complaint rate for this antibody, and we had only 9complaints in over 2 years with over 150 units sold. therefore, i think this is usually a ...

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