Mouse monoclonal [MYSN02] to Fast Myosin Skeletal Heavy chain, prediluted
Specificityab75370 may be used for detecting cross striated muscle differentiation in tumors. It will not stain human or animal cardiac muscle, smooth muscle myosin, or cells grown by tissue culture (non muscle myosin).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Ready to use.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Sequence similaritiesContains 1 IQ domain. Contains 1 myosin head-like domain.
DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
Anti-Fast Myosin Skeletal Heavy chain antibody [MYSN02], prediluted images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fast Myosin Skeletal Heavy chain antibody [MYSN02 ], prediluted (ab75370)
ab75370 staining Fast Myosin Skeletal Heavy chain in formalin fixed, paraffin embedded human skeletal muscle tissue by Immunohistochemistry, using peroxidase conjugate and AEC chromogen. Note cytoplasmic staining of muscle cells.
References for Anti-Fast Myosin Skeletal Heavy chain antibody [MYSN02], prediluted (ab75370)
This product has been referenced in:
Lundgreen K et al. Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears. Acta Orthop84:565-70 (2013).
Read more (PubMed: 24171689) »