Anti-fast skeletal Myosin antibody [Y32], prediluted (ab909)

Overview

  • Product nameAnti-fast skeletal Myosin antibody [Y32], prediluted
    See all fast skeletal Myosin primary antibodies
  • Description
    Mouse monoclonal [Y32] to fast skeletal Myosin, prediluted
  • SpecificityThis antibody recognizes heavy chain of human skeletal myosin. It does not stain cardiac or smooth muscle myosin. This antibody stains the fast twitch (type II) isomyosin molecule.
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Human
  • Immunogen

    Full length protein (Rabbit).

  • Positive control
    • IHC-P: FFPE human skeletal msucle tissue sections.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: Inert stabilizer
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone numberY32
  • Myelomaunknown
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
  • Application notesThis antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
  • Target

    Anti-fast skeletal Myosin antibody [Y32], prediluted images

    • IHC image of fast skeletal Myosin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab909, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

    References for Anti-fast skeletal Myosin antibody [Y32], prediluted (ab909)

    This product has been referenced in:
    • Haider KH  et al. MicroRNA-21 is a key determinant in IL-11/Stat3 anti-apoptotic signalling pathway in preconditioning of skeletal myoblasts. Cardiovasc Res 88:168-78 (2010). IHC-Fr ; Rat . Read more (PubMed: 20498256) »
    • Barker T  et al. Vitamin E and C supplementation does not ameliorate muscle dysfunction after anterior cruciate ligament surgery. Free Radic Biol Med 47:1611-8 (2009). IHC-P ; Human . Read more (PubMed: 19751822) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"