Anti-FASTK antibody (ab97544)
Key features and details
- Rabbit polyclonal to FASTK
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FASTK antibody -
Description
Rabbit polyclonal to FASTK -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig -
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 396-521 of Human FASTK (NP_006703)
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Positive control
- WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji or NIH3T3 cell lysates ICC/IF: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor. -
Tissue specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the FAST protein kinase family.
Contains 1 RAP domain. -
Post-translational
modificationsAutophosphorylated on serine/threonine residues. Activated by dephosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 509781 Cow
- Entrez Gene: 482802 Dog
- Entrez Gene: 10922 Human
- Entrez Gene: 66587 Mouse
- Entrez Gene: 296741 Rat
- Omim: 606965 Human
- SwissProt: Q14296 Human
- SwissProt: Q9JIX9 Mouse
see all -
Alternative names
- Fas activated serine threonine kinase antibody
- Fas-activated serine/threonine kinase antibody
- FAST antibody
see all
Images
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All lanes : Anti-FASTK antibody (ab97544) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : A375 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa -
Anti-FASTK antibody (ab97544) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg
Predicted band size: 61 kDa -
Anti-FASTK antibody (ab97544) at 1/1000 dilution + NIH-3T3 cell lysate at 30 µg
Predicted band size: 61 kDa -
Immunofluorescent staining of FASTK in HeLa cells using ab97544 at a dilution of 1/1000. HeLa cells were fixed in ice-cold methanol for 5 minutes and the cells were counter-stained with Hoechst 33342.
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FASTK siRNA interference assay. Three siRNA sequences targeting different sites of human FASTK cDNA and a scrambled control siRNA (si-NC) were transfected into U251 cells using Lipofectamine 2000. Total protein or total RNA was isolated at 48 h or 24 h post-transfection. FASTK protein levels were determined by western blot analysis using ab97544 at a dilution of 1/500 (A). The secondary antibody was a rabbit IgG-HRP and the quantitive data was obtained with the Quantity One analysis program. FASTK mRNA levels were assessed by qRT-PCR (B).
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Immunofluorescence analysis of FASTK in methanol fixed HeLa, using ab97544 at a 1/200 dilution.
Lower image is co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab97544 has been referenced in 2 publications.
- Ohkubo A et al. The FASTK family proteins fine-tune mitochondrial RNA processing. PLoS Genet 17:e1009873 (2021). PubMed: 34748562
- Zhi F et al. miR-106a-5p inhibits the proliferation and migration of astrocytoma cells and promotes apoptosis by targeting FASTK. PLoS One 8:e72390 (2013). WB . PubMed: 24013584