Overview

  • Product name
  • Description
    Rabbit polyclonal to FASTK
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 396-521 of Human FASTK (NP_006703)

  • Positive control
    • WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji or NIH3T3 cell lysates ICC/IF: HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/200.

Target

Images

  • All lanes : Anti-FASTK antibody (ab97544) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate
    Lane 5 : A375 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 61 kDa
  • Anti-FASTK antibody (ab97544) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg

    Predicted band size : 61 kDa
  • Anti-FASTK antibody (ab97544) at 1/1000 dilution + NIH-3T3 cell lysate at 30 µg

    Predicted band size : 61 kDa
  • Immunohistochemical staining of paraffin-embedded SCC15 xenograft, using ab97544 at 1/100 dilution.

  • Immunofluorescent staining of FASTK in HeLa cells using ab97544 at a dilution of 1/1000. HeLa cells were fixed in ice-cold methanol for 5 minutes and the cells were counter-stained with Hoechst 33342.

  • FASTK siRNA interference assay. Three siRNA sequences targeting different sites of human FASTK cDNA and a scrambled control siRNA (si-NC) were transfected into U251 cells using Lipofectamine 2000. Total protein or total RNA was isolated at 48 h or 24 h post-transfection. FASTK protein levels were determined by western blot analysis using ab97544 at a dilution of 1/500 (A). The secondary antibody was a rabbit IgG-HRP and the quantitive data was obtained with the Quantity One analysis program. FASTK mRNA levels were assessed by qRT-PCR (B).

  • All lanes : Anti-FASTK antibody (ab97544) at 1/500 dilution

    Lane 1 : MOLT4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 61 kDa
    12% SDS PAGE
  • Immunofluorescence analysis of FASTK in methanol fixed HeLa, using ab97544 at a 1/200 dilution.
    Lower image is co-stained with Hoechst 33342.

References

This product has been referenced in:
  • Zhi F  et al. miR-106a-5p inhibits the proliferation and migration of astrocytoma cells and promotes apoptosis by targeting FASTK. PLoS One 8:e72390 (2013). WB . Read more (PubMed: 24013584) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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