Overview

  • Product nameAnti-FATP1 antibody
    See all FATP1 primary antibodies
  • Description
    Rabbit polyclonal to FATP1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human SLC27A1 .

  • Positive control
    • This antibody gave a positive signal in Human Skeletal Muscle and Human Liver Tissue Lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab81875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 71 kDa).

Target

  • RelevanceSLC27A1 (Solute carrier family 27 member 1) is involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. SLC27A1 may act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. It also plays a pivotal role in regulating available LFCA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis and may be involved in regulation of cholesterol metabolism.
  • Cellular localizationCell membrane; Single-pass membrane protein. Intracytoplasmic membrane; Single-pass membrane protein. Cytoplasm. Note=Plasma membrane and intracellular membranes, at least in adipocytes. Predominantly cytoplasmic in myocytes.
  • Database links
  • Alternative names
    • ACSVL5 antibody
    • FATP 1 antibody
    • FATP antibody
    • FATP1 antibody
    • Fatty acid transport protein 1 antibody
    • FLJ00336 antibody
    • Long chain fatty acid transport protein 1 antibody
    • MGC71751 antibody
    • SLC27A1 antibody
    • Solute carrier family 27 (fatty acid transporter) member 1 antibody
    • Solute carrier family 27 member 1 antibody
    see all

Anti-FATP1 antibody images

  • All lanes : Anti-FATP1 antibody (ab81875) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330) with Immunising Peptide at 1 µg/ml
    Lane 4 : Human liver tissue lysate - total protein (ab29889) with Immunising Peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab81875 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab81875 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in This antibody also gave a positive result in Hek293, HepG2, and MCF-7 formaldehyde (4%, 10min) fixed cells at 1ug/ml, and also in HeLa, Hek293, HepG2, and MCF-7 Methanol (100%, 5min) fixed cells at 1ug/ml.

References for Anti-FATP1 antibody (ab81875)

ab81875 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"