The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-FATP1 antibody (ab81875)
70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Fatty acid transport protein 1
Long chain fatty acid transport protein 1
Solute carrier family 27 (fatty acid transporter) member 1
Solute carrier family 27 member 1
SLC27A1 (Solute carrier family 27 member 1) is involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. SLC27A1 may act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. It also plays a pivotal role in regulating available LFCA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis and may be involved in regulation of cholesterol metabolism.
Cell membrane; Single-pass membrane protein. Intracytoplasmic membrane; Single-pass membrane protein. Cytoplasm. Note=Plasma membrane and intracellular membranes, at least in adipocytes. Predominantly cytoplasmic in myocytes.
References for FATP1 peptide (ab104213)
has not yet been referenced specifically in any publications.
Publishing research using ab104213? Please let us know so that we can cite the reference in this datasheet.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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