Anti-Fatty Acid Binding Protein 5 antibody (ab84028)

Overview

  • Product name
    Anti-Fatty Acid Binding Protein 5 antibody
    See all Fatty Acid Binding Protein 5 primary antibodies
  • Description
    Rabbit polyclonal to Fatty Acid Binding Protein 5
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Fatty Acid Binding Protein 5.

    (Peptide available as ab94388.)

  • Positive control
    • This antibody gave a positive signal in the following human lysates: skin tissue; HeLa whole cell; HepG2 whole cell; A431 whole cell.

Properties

Applications

Our Abpromise guarantee covers the use of ab84028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).

Target

  • Function
    High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation.
  • Tissue specificity
    Keratinocytes; highly expressed in psoriatic skin.
  • Sequence similarities
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain
    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFABP antibody
    • Cutaneous fatty acid binding protein antibody
    • DA11 antibody
    • Differentiation associated lipid binding protein LP2 antibody
    • E-FABP antibody
    • EFABP antibody
    • Epidermal-type fatty acid-binding protein antibody
    • FABP5 antibody
    • FABP5_HUMAN antibody
    • fatty acid binding protein 5 (psoriasis-associated) antibody
    • Fatty acid binding protein epidermal antibody
    • Fatty acid-binding protein 5 antibody
    • Fatty acid-binding protein, epidermal antibody
    • Keratinocyte lipid binding protein antibody
    • KFABP antibody
    • Klbp antibody
    • mal1 antibody
    • PA-FABP antibody
    • PAFABP antibody
    • Psoriasis associated fatty acid binding protein homolog antibody
    • Psoriasis-associated fatty acid-binding protein homolog antibody
    see all

Images

  • Fatty Acid Binding Protein 5 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84028.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 16kDa; Fatty Acid Binding Protein 5

  • All lanes : Anti-Fatty Acid Binding Protein 5 antibody (ab84028) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • ICC/IF image of ab84028 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84028, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, MCF-7 cells at 5µg/ml.

References

This product has been referenced in:
  • Suojalehto H  et al. Level of Fatty Acid Binding Protein 5 (FABP5) Is Increased in Sputum of Allergic Asthmatics and Links to Airway Remodeling and Inflammation. PLoS One 10:e0127003 (2015). Read more (PubMed: 26020772) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Purified protein (Protein found within commercial Milk Fat Globule M)
Gel Running Conditions
Non-reduced Denaturing (15% gel)
Loading amount
1 µg
Specification
Protein found within commercial Milk Fat Globule M
Blocking step
Seablock - thermo fisher as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C
Username

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Submitted Nov 02 2017

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