The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
FunctionHigh specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation.
Tissue specificityKeratinocytes; highly expressed in psoriatic skin.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
Differentiation associated lipid binding protein LP2 antibody
Epidermal-type fatty acid-binding protein antibody
fatty acid binding protein 5 (psoriasis-associated) antibody
Fatty acid binding protein epidermal antibody
Fatty acid-binding protein 5 antibody
Fatty acid-binding protein, epidermal antibody
Keratinocyte lipid binding protein antibody
Psoriasis associated fatty acid binding protein homolog antibody
Psoriasis-associated fatty acid-binding protein homolog antibody
Anti-Fatty Acid Binding Protein 5 antibody images
Immunoprecipitation - Anti-Fatty Acid Binding Protein 5 antibody (ab84028)
Fatty Acid Binding Protein 5 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84028.
Immunocytochemistry/ Immunofluorescence - Fatty Acid Binding Protein 5 antibody (ab84028)
ICC/IF image of ab84028 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84028, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, MCF-7 cells at 5µg/ml.
References for Anti-Fatty Acid Binding Protein 5 antibody (ab84028)
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