Anti-Fatty Acid Synthase antibody (ab99359)


  • Product nameAnti-Fatty Acid Synthase antibody
    See all Fatty Acid Synthase primary antibodies
  • Description
    Rabbit polyclonal to Fatty Acid Synthase
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster
  • Immunogen

    A region within amino acids 2462-2511 of Human Fatty Acid Synthase (NP_004095.4).

  • Positive control
    • HeLa whole cell lysate. 293T cells. IF/ICC: A549 cell line. IHC-P: FFPE human breast adenocarcinoma tissue sections.

Associated products


Our Abpromise guarantee covers the use of ab99359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 273 kDa.
IP Use at 2-5 µg/mg of lysate.


  • FunctionFatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
  • Tissue specificityUbiquitous. Prominent expression in brain, lung, and liver.
  • Sequence similaritiesContains 1 acyl carrier domain.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

Anti-Fatty Acid Synthase antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling Fatty Acid Synthase with ab99359 at 1/200 (1µg/ml). Detection: DAB.
  • IHC image of Fatty Acid Synthase staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99359, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab99359 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab99359, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Fatty Acid Synthase antibody (ab99359) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 273 kDa

    Exposure time : 30 seconds
  • ab99359 at 0.1 µg/ml detecting Fatty Acid Synthase in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 30 second exposure.
    For immunoprecipitation, ab99359 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99358 (a rabbit anti-Fatty Acid Synthase antibody which recognizes a upstream epitope).
    Lane 2; IP using ab99359.
    Lane 3; IP using control IgG.

References for Anti-Fatty Acid Synthase antibody (ab99359)

ab99359 has not yet been referenced specifically in any publications.

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