Overview

  • Product nameAnti-FAU antibody
    See all FAU primary antibodies
  • Description
    Rabbit polyclonal to FAU
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the N-terminal region of human FAU.

  • Positive control
    • 293 cell lysate or human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab63063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Sequence similaritiesBelongs to the ubiquitin family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40S ribosomal protein S30 antibody
    • asr1 antibody
    • FAU antibody
    • FAU encoded ubiquitin like protein antibody
    • FAU1 antibody
    • FBR MuSV associated ubiquitously expressed antibody
    • Finkel Biskis Reilly murine sarcoma virus (FBR MuSV) ubiquitously expressed (fox derived) antibody
    • Finkel Biskis Reilly murine sarcoma virus (FBR MuSV) ubiquitously expressed antibody
    • Finkel Biskis Reilly murine sarcoma virus ubiquitously expressed antibody
    • FLJ22986 antibody
    • Fub1 antibody
    • Fubi antibody
    • MNSFbeta antibody
    • Monoclonal nonspecific suppressor factor beta antibody
    • Ribosomal protein S30 antibody
    • RPS30 antibody
    • S30 antibody
    • UBIM_HUMAN antibody
    • Ubiquitin like protein fubi and ribosomal protein S30 antibody
    • Ubiquitin like protein fubi antibody
    • Ubiquitin like S30 fusion protein antibody
    • Ubiquitin-like protein FUBI antibody
    see all

Anti-FAU antibody images

  • All lanes : Anti-FAU antibody (ab63063) at 1/100 dilution

    Lane 1 : 293 cell lysate, nontransfected
    Lane 2 : 293 cell lysate, transiently transfected with the FAU gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 14 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  • Ab63063 (1/50-1/100) staining human FAU in human colon carcinoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    Ab63063 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References for Anti-FAU antibody (ab63063)

ab63063 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 7.5 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Jul 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (U20S)
Specification U20S
Permeabilization Yes - Triton-X
Fixative Paraformaldehyde
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Submitted Jul 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"