Overview

  • Product nameAnti-FAU antibody
    See all FAU primary antibodies
  • Description
    Rabbit polyclonal to FAU
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C-terminal region of human FAU.

  • Positive control
    • Human colon carcinoma tissue, Hela and T47D cell line lysates and 293 cell line lysates transiently transfected with the FAU gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab63065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Sequence similaritiesBelongs to the ubiquitin family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40S ribosomal protein S30 antibody
    • asr1 antibody
    • FAU antibody
    • FAU encoded ubiquitin like protein antibody
    • FAU1 antibody
    • FBR MuSV associated ubiquitously expressed antibody
    • Finkel Biskis Reilly murine sarcoma virus (FBR MuSV) ubiquitously expressed (fox derived) antibody
    • Finkel Biskis Reilly murine sarcoma virus (FBR MuSV) ubiquitously expressed antibody
    • Finkel Biskis Reilly murine sarcoma virus ubiquitously expressed antibody
    • FLJ22986 antibody
    • Fub1 antibody
    • Fubi antibody
    • MNSFbeta antibody
    • Monoclonal nonspecific suppressor factor beta antibody
    • Ribosomal protein S30 antibody
    • RPS30 antibody
    • S30 antibody
    • UBIM_HUMAN antibody
    • Ubiquitin like protein fubi and ribosomal protein S30 antibody
    • Ubiquitin like protein fubi antibody
    • Ubiquitin like S30 fusion protein antibody
    • Ubiquitin-like protein FUBI antibody
    see all

Anti-FAU antibody images

  • ab63065 at 1/50 - 1/100 dilution, staining FAU in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
  • Anti-FAU antibody (ab63065) at 1/60 dilution + HeLa cell lysate at 2µg per lane

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • Anti-FAU antibody (ab63065) at 1/60 dilution + T47D cell lysate at 2µg per lane

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • All lanes : Anti-FAU antibody (ab63065) at 1/60 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the FAU gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 14 kDa
    Observed band size : 14 kDa

References for Anti-FAU antibody (ab63065)

ab63065 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 7.5 µg
Gel Running Conditions Reduced Denaturing (4-12% bis-Tris)
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Jul 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (U2OS)
Specification U2OS
Permeabilization Yes - Triton-X
Fixative Paraformaldehyde
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Verified customer

Submitted Jul 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"