- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-FAU antibody
See all FAU primary antibodies -
DescriptionRabbit polyclonal to FAU
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
KLH conjugated synthetic peptide selected from the C-terminal region of human FAU.
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Positive control
- Human colon carcinoma tissue, Hela and T47D cell line lysates and 293 cell line lysates transiently transfected with the FAU gene.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityProtein G purified
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Purification notesab63065 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab63065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50 - 1/100. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). | |
| ELISA | 1/1000. | |
| IHC-P | 1/50 - 1/100. |
Target
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Sequence similaritiesBelongs to the ubiquitin family.
- Information by UniProt
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Database links
- Entrez Gene: 2197 Human
- Omim: 134690 Human
- SwissProt: P35544 Human
- Unigene: 387208 Human
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Alternative names
- 40S ribosomal protein S30 antibody
- asr1 antibody
- FAU antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAU antibody (ab63065)ab63065 at 1/50 - 1/100 dilution, staining FAU in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining. -
Western blot - Anti-FAU antibody (ab63065)Anti-FAU antibody (ab63065) at 1/60 dilution + HeLa cell lysate at 2µg per lane
Predicted band size: 14 kDa
Observed band size: 14 kDa -
Western blot - Anti-FAU antibody (ab63065)Anti-FAU antibody (ab63065) at 1/60 dilution + T47D cell lysate at 2µg per lane
Predicted band size: 14 kDa
Observed band size: 14 kDa -
Western blot - Anti-FAU antibody (ab63065)All lanes : Anti-FAU antibody (ab63065) at 1/60 dilution
Lane 1 : 293 cell lysate
Lane 2 : 293 cell lysate transiently transfected with the FAU gene
Lysates/proteins at 2 µg per lane.
Predicted band size: 14 kDa
Observed band size: 14 kDa
References
ab63065 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"