Customer reviews and FAQs

Overview

  • Product name
    Anti-FAU antibody
    See all FAU primary antibodies
  • Description
    Rabbit polyclonal to FAU
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C-terminal region of human FAU.

  • Positive control
    • Human colon carcinoma tissue, Hela and T47D cell line lysates and 293 cell line lysates transiently transfected with the FAU gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab63065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Sequence similarities
    Belongs to the ubiquitin family.
  • Information by UniProt
  • Database links
    • Alternative names
      • 40S ribosomal protein S30 antibody
      • asr1 antibody
      • FAU antibody
      • FAU encoded ubiquitin like protein antibody
      • FAU1 antibody
      • FBR MuSV associated ubiquitously expressed antibody
      • Finkel Biskis Reilly murine sarcoma virus (FBR MuSV) ubiquitously expressed (fox derived) antibody
      • Finkel Biskis Reilly murine sarcoma virus (FBR MuSV) ubiquitously expressed antibody
      • Finkel Biskis Reilly murine sarcoma virus ubiquitously expressed antibody
      • FLJ22986 antibody
      • Fub1 antibody
      • Fubi antibody
      • MNSFbeta antibody
      • Monoclonal nonspecific suppressor factor beta antibody
      • Ribosomal protein S30 antibody
      • RPS30 antibody
      • S30 antibody
      • UBIM_HUMAN antibody
      • Ubiquitin like protein fubi and ribosomal protein S30 antibody
      • Ubiquitin like protein fubi antibody
      • Ubiquitin like S30 fusion protein antibody
      • Ubiquitin-like protein FUBI antibody
      see all

    Anti-FAU antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAU antibody (ab63065)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FAU antibody (ab63065)
      ab63065 at 1/50 - 1/100 dilution, staining FAU in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
    • Western blot - FAU antibody (ab63065)
      Western blot - FAU antibody (ab63065)
      Anti-FAU antibody (ab63065) at 1/60 dilution + HeLa cell lysate at 2µg per lane

      Predicted band size : 14 kDa
      Observed band size : 14 kDa
    • Western blot - FAU antibody (ab63065)
      Western blot - FAU antibody (ab63065)
      Anti-FAU antibody (ab63065) at 1/60 dilution + T47D cell lysate at 2µg per lane

      Predicted band size : 14 kDa
      Observed band size : 14 kDa
    • Western blot - FAU antibody (ab63065)
      Western blot - FAU antibody (ab63065)
      All lanes : Anti-FAU antibody (ab63065) at 1/60 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : 293 cell lysate transiently transfected with the FAU gene

      Lysates/proteins at 2 µg per lane.


      Predicted band size : 14 kDa
      Observed band size : 14 kDa

    References for Anti-FAU antibody (ab63065)

    ab63065 has not yet been referenced specifically in any publications.

    Publishing research using ab63065? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab63065 Submit a question for ab63065

    2 Abreviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-FAU antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    7.5 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% bis-Tris)
    Sample
    Human Cell lysate - whole cell (U2OS)
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
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    Abcam user community

    Verified customer

    Submitted Jul 22 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FAU antibody

     Inconclusive
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample
    Human Cell (U2OS)
    Specification
    U2OS
    Permeabilization
    Yes - Triton-X
    Fixative
    Paraformaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Jul 02 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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