Overview

  • Product nameAnti-FAU antibody
    See all FAU primary antibodies
  • Description
    Rabbit polyclonal to FAU
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the center region of human FAU.

  • Positive control
    • Lysate from HeLa or T47D cell line, or human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab63066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Sequence similaritiesBelongs to the ubiquitin family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40S ribosomal protein S30 antibody
    • asr1 antibody
    • FAU antibody
    • FAU encoded ubiquitin like protein antibody
    • FAU1 antibody
    • FBR MuSV associated ubiquitously expressed antibody
    • Finkel Biskis Reilly murine sarcoma virus (FBR MuSV) ubiquitously expressed (fox derived) antibody
    • Finkel Biskis Reilly murine sarcoma virus (FBR MuSV) ubiquitously expressed antibody
    • Finkel Biskis Reilly murine sarcoma virus ubiquitously expressed antibody
    • FLJ22986 antibody
    • Fub1 antibody
    • Fubi antibody
    • MNSFbeta antibody
    • Monoclonal nonspecific suppressor factor beta antibody
    • Ribosomal protein S30 antibody
    • RPS30 antibody
    • S30 antibody
    • UBIM_HUMAN antibody
    • Ubiquitin like protein fubi and ribosomal protein S30 antibody
    • Ubiquitin like protein fubi antibody
    • Ubiquitin like S30 fusion protein antibody
    • Ubiquitin-like protein FUBI antibody
    see all

Anti-FAU antibody images

  • Anti-FAU antibody (ab63066) at 1/250 dilution + T47D cell lysate at 12.5 µg

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : ~30 kDa,~65 kDa,~75 kDa. We are unsure as to the identity of these extra bands.
  • Anti-FAU antibody (ab63066) at 1/250 dilution + HeLa cell lysate at 12.5 µg

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : 40-80 kDa. We are unsure as to the identity of these extra bands.
  • Ab63066 (1/50-1/100) staining human FAU in human colon carcinoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    Ab63066 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References for Anti-FAU antibody (ab63066)

ab63066 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 7.5 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Jul 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U20S)
Specification U20S
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
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Submitted Jul 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"