Overview

  • Product nameAnti-FAU antibody
    See all FAU primary antibodies
  • Description
    Rabbit polyclonal to FAU
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide: VRGQTPKVAK QEKKKKKTGR AKRRMQYNRR FVNVVPTFGK KKGPNANS, corresponding to internal sequence amino acids 85-134 of Human FAU (NP_001988).

  • Positive control
    • 721_B cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab81442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-FAU antibody images



  • Predicted band size : 14 kDa
    Western blot analysis of MCF7 cell lysate labeling FAU with ab81442 at 1.0µg/ml.


  • Predicted band size : 14 kDa
    Western blot analysis of Jurkat cell lysate labeling FAU with ab81442 at 1.0µg/ml.


  • Predicted band size : 14 kDa
    Western blot analysis of HeLa cell lysate labeling FAU with ab81442 at 1.0µg/ml.
  • Anti-FAU antibody (ab81442) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Gel concentration 10-20%

References for Anti-FAU antibody (ab81442)

ab81442 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 7.5 µg
Gel Running Conditions Reduced Denaturing (4-12% bis-Tris)
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Jul 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (U20S)
Specification U20S
Permeabilization Yes - Triton-X
Fixative Paraformaldehyde
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Submitted Jul 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"