The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RelevanceKeratin-binding protein required for epithelial cell polarization. Involved in apical junction complex (AJC) assembly via its interaction with PARD3. Required for ciliogenesis.
Cellular localizationCytoplasm; cytoskeleton; centrosome; centriole. Cytoplasm; cytoskeleton; spindle pole. Cell junction. Note: Associates with the mother centriole. Localizes to the apical junction complex (AJC) in epithelial cells