Overview

  • Product nameAnti-FBLIM1 antibody
    See all FBLIM1 primary antibodies
  • Description
    Rabbit polyclonal to FBLIM1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide between 301-331 amino acids from the C-terminal region of Human FBLIM1 (NP_001019387.1 , NP_001019386.1 , NP_060026.2).

  • Positive control
    • A549 cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab112590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 41 kDa.

Target

  • FunctionServes as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin-containing signaling complexes that control actin assembly.
  • Tissue specificityIsoform 1 and isoform 3 are expressed in heart, kidney, lung, pancreas, placenta and platelets. Isoform 2 is expressed in brain, heart, kidney, lung, pancreas, placenta, skeletal muscle and platelets.
  • Sequence similaritiesContains 3 LIM zinc-binding domains.
  • Cellular localizationCytoplasm > cell cortex. Cell junction > focal adhesion. Cytoplasm > cytoskeleton. Associated with actin stress fiber at cell-ECM focal adhesion sites. Isoform 1 and isoform 3 are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. Isoform 2 is localized at actin stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410043F08Rik antibody
    • Cal antibody
    • CSX associated LIM antibody
    • DKFZp434G171 antibody
    • FBLI1_HUMAN antibody
    • FBLIM 1 antibody
    • Fblim1 antibody
    • FBLP 1 antibody
    • FBLP-1 antibody
    • FBLP1 antibody
    • Filamin binding LIM protein 1 antibody
    • Filamin-binding LIM protein 1 antibody
    • Gt10 antibody
    • MGC105665 antibody
    • MIG2 interacting protein; antibody
    • MIG2-interacting protein antibody
    • Migfilin antibody
    • Mitogen inducible 2 interacting protein antibody
    • Mitogen-inducible 2-interacting protein antibody
    • OTTHUMP00000003118 antibody
    • OTTHUMP00000003119 antibody
    • OTTHUMP00000003120 antibody
    • OTTMUSP00000011062 antibody
    • OTTMUSP00000011063 antibody
    • RP11-169K16.5 antibody
    • RP23-291H3.1 antibody
    see all

Anti-FBLIM1 antibody images

  • Anti-FBLIM1 antibody (ab112590) at 1/100 dilution + A549 cell line lysate at 35 µg

    Predicted band size : 41 kDa

References for Anti-FBLIM1 antibody (ab112590)

ab112590 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification Mouse Embryonic Fibroblast (MEF)
Fixative Paraformaldehyde
Permeabilization Yes - triton
Username

Abcam user community

Verified customer

Submitted Nov 16 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cell line ( cervical cancer))
Specification HeLa cell line ( cervical cancer)
Fixative Paraformaldehyde
Permeabilization Yes - triton
Username

Abcam user community

Verified customer

Submitted Nov 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"