Overview

  • Product nameAnti-FBP1 antibody
    See all FBP1 primary antibodies
  • Description
    Rabbit polyclonal to FBP1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1 - 338 of Human FBP1 (NP_000498.2).

  • Positive control
    • Mouse liver tissue lysate and transfected 293T cell lysate IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab103165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • PathwayCarbohydrate biosynthesis; gluconeogenesis.
  • Involvement in diseaseDefects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) [MIM:229700]. FBPD is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
  • Sequence similaritiesBelongs to the FBPase class 1 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6-bisphosphatase 1 antibody
    • 6-bisphosphate 1-phosphohydrolase 1 antibody
    • D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody
    • D-fructose-1 antibody
    • EC 3.1.3.11 antibody
    • F16P1_HUMAN antibody
    • FBP antibody
    • FBP 1 antibody
    • FBP1 antibody
    • FBPase 1 antibody
    • Fructose 1 6 bisphosphatase 1 antibody
    • Fructose bisphosphatase 1 antibody
    • Fructose-1 antibody
    • Growth inhibiting protein 17 antibody
    • Liver fructose bisphosphatase antibody
    see all

Anti-FBP1 antibody images

  • ICC/IF image of ab103165 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103165, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FBP1 antibody (ab103165) at 1 µg/ml + mouse liver tissue lysate at 50 µg

    Predicted band size : 37 kDa
  • All lanes : Anti-FBP1 antibody (ab103165) at 1 µg/ml

    Lane 1 : FBP1 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 37 kDa

References for Anti-FBP1 antibody (ab103165)

ab103165 has not yet been referenced specifically in any publications.

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