Overview

  • Product nameAnti-FBP1 antibody [EPR4619]
    See all FBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4619] to FBP1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human FBP1 aa 50-150.

  • Positive control
    • HL60 lysate treated with vitamin D3, MCF7 and Human fetal liver lysates; Human kidney and liver tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/50 - 1/100. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for ICC/IF.
  • Target

    • PathwayCarbohydrate biosynthesis; gluconeogenesis.
    • Involvement in diseaseDefects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) [MIM:229700]. FBPD is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
    • Sequence similaritiesBelongs to the FBPase class 1 family.
    • Information by UniProt
    • Database links
    • Alternative names
      • 6-bisphosphatase 1 antibody
      • 6-bisphosphate 1-phosphohydrolase 1 antibody
      • D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody
      • D-fructose-1 antibody
      • EC 3.1.3.11 antibody
      • F16P1_HUMAN antibody
      • FBP antibody
      • FBP 1 antibody
      • FBP1 antibody
      • FBPase 1 antibody
      • Fructose 1 6 bisphosphatase 1 antibody
      • Fructose bisphosphatase 1 antibody
      • Fructose-1 antibody
      • Growth inhibiting protein 17 antibody
      • Liver fructose bisphosphatase antibody
      see all

    Anti-FBP1 antibody [EPR4619] images

    • ab109020 at 1:500 staining Anti-FBP1 antibody in mouse kidney tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling FBP1 with ab109020 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.

    • All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/10000 dilution (purified)

      Lane 1 : Mouse liver
      Lane 2 : Rat liver

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 37 kDa
      Observed band size : 37 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/2000 dilution (purified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : HL-60 cell lysate treated with vitamin D3

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/2000 dilution

      Predicted band size : 37 kDa
      Observed band size : 37 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • ab109020 at 1:500 staining Anti-FBP1 antibody in human liver tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FBP1 with ab109020 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.

    • All lanes : Anti-FBP1 antibody [EPR4619] (ab109020) at 1/1000 dilution (unpurified)

      Lane 1 : MCF7 lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : HL60 lysate, treated with vitamin D3

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 37 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Unpurified ab109020 at 1/50 dilution staining FBP1 in Human Kidney by immunohistochemistry, paraffin-embedded tissue.

    • Unpurified ab109020 at 1/50 dilution staining FBP1 in Human liver by immunohistochemistry, paraffin-embedded tissue.

    References for Anti-FBP1 antibody [EPR4619] (ab109020)

    This product has been referenced in:
    • Shi S  et al. Metabolic tumor burden is associated with major oncogenomic alterations and serum tumor markers in patients with resected pancreatic cancer. Cancer Lett 360:227-33 (2015). WB ; Human . Read more (PubMed: 25687883) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"