Overview

  • Product nameAnti-FBP1 antibody [EPR4620]
    See all FBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4620] to FBP1
  • Tested applicationsSuitable for: ICC/IF, WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human FBP1.

  • Positive control
    • Human stomach, Human fetal liver, and MCF7 lysates IF/ICC: HeLa cell line
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-FBP1 antibody (Alexa Fluor® 488) [EPR4620] (ab206716)

    Anti-FBP1 antibody (Alexa Fluor® 647) [EPR4620] (ab207051)

Associated products

Applications

Our Abpromise guarantee covers the use of ab109732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • PathwayCarbohydrate biosynthesis; gluconeogenesis.
    • Involvement in diseaseDefects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) [MIM:229700]. FBPD is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
    • Sequence similaritiesBelongs to the FBPase class 1 family.
    • Information by UniProt
    • Database links
    • Alternative names
      • 6-bisphosphatase 1 antibody
      • 6-bisphosphate 1-phosphohydrolase 1 antibody
      • D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody
      • D-fructose-1 antibody
      • EC 3.1.3.11 antibody
      • F16P1_HUMAN antibody
      • FBP antibody
      • FBP 1 antibody
      • FBP1 antibody
      • FBPase 1 antibody
      • Fructose 1 6 bisphosphatase 1 antibody
      • Fructose bisphosphatase 1 antibody
      • Fructose-1 antibody
      • Growth inhibiting protein 17 antibody
      • Liver fructose bisphosphatase antibody
      see all

    Anti-FBP1 antibody [EPR4620] images

    • ICC/IF image of ab109732 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109732, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution

      Lane 1 : Human stomach lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : MCF7 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 37 kDa

    References for Anti-FBP1 antibody [EPR4620] (ab109732)

    This product has been referenced in:
    • Du Y  et al. Lysine malonylation is elevated in type 2 diabetic mouse models and enriched in metabolic associated proteins. Mol Cell Proteomics 14:227-36 (2015). Read more (PubMed: 25418362) »
    • Cyr AR  et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24469049) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"