Recombinant Anti-FBP1 + FBP2 antibody [EPR7435] (ab131333)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7435] to FBP1 + FBP2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FBP1 + FBP2 antibody [EPR7435] -
Description
Rabbit monoclonal [EPR7435] to FBP1 + FBP2 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 93% homology with FBP1. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal kidney, Human skeletal muscle and fetal liver lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.17 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7435 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 37 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 37 kDa). |
Target
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Cellular localization
FBP2: Cell junction. Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. In neonatal cardiomyocytes, distributed throughout the cytosol, accumulating in the intercalated disks which occur at the Z line of cardiomyocytes and connect adjacent cells, and also located in the nucleus; dissociates from the Z line following an increase in cytosolic Ca(2+) concentration (By similarity). In muscle precursor cells, localizes predominantly to the nucleus and to a lesser extent to the cytoplasm at the proliferative phase, while mainly localizing to the cytoplasm at the differentiation phase (By similarity). Colocalizes with ALDOA and alpha-actinin on both sides of the Z line of skeletal muscle; dissociates rapidly from the Z line following an increase in cytosolic Ca(2+) concentration. -
Database links
- Entrez Gene: 2203 Human
- Entrez Gene: 8789 Human
- Entrez Gene: 14120 Mouse
- Entrez Gene: 14121 Mouse
- Entrez Gene: 114508 Rat
- Entrez Gene: 24362 Rat
- Omim: 603027 Human
- Omim: 611570 Human
see all
Images
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All lanes : Anti-FBP1 + FBP2 antibody [EPR7435] (ab131333) at 1/10000 dilution
Lane 1 : C-Myc/DDK-tagged human FBP1 full length recombinant protein
Lane 2 : C-Myc/DDK-tagged human FBP2 full length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 20 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-FBP1 + FBP2 antibody [EPR7435] (ab131333) at 1/1000 dilution
Lane 1 : Fetal kidney lysates
Lane 2 : Human skeletal muscle lysates
Lane 3 : Fetal liver lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 37 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131333 has not yet been referenced specifically in any publications.