Overview

  • Product nameAnti-Fbx32 antibody
    See all Fbx32 primary antibodies
  • Description
    Mouse polyclonal to Fbx32
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Fbx32 protein (Human)

  • Positive control
    • Fbx32 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab67866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. PubMed: 20065949
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 42 kDa).

Target

  • FunctionSubstrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
  • Tissue specificitySpecifically expressed in cardiac and skeletal muscle.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 F-box domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4833442G10Rik antibody
    • AI430017 antibody
    • Atrogin 1 antibody
    • Atrogin-1 antibody
    • ATROGIN1 antibody
    • Atrophy gene 1 antibody
    • F box only protein 32 antibody
    • F-box only protein 32 antibody
    • F-box protein 32 antibody
    • FBX32_HUMAN antibody
    • fbxo25 antibody
    • FBXO32 antibody
    • FLJ32424 antibody
    • MAFbx antibody
    • MGC108443 antibody
    • MGC137646 antibody
    • MGC33610 antibody
    • Muscle atrophy F box antibody
    • Muscle atrophy F box protein antibody
    • Muscle atrophy F-box protein antibody
    see all

Anti-Fbx32 antibody images

  • All lanes : Anti-Fbx32 antibody (ab67866) at 1/500 dilution

    Lane 1 : Fbx32 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 42 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References for Anti-Fbx32 antibody (ab67866)

This product has been referenced in:
  • MacNeil LG  et al. The order of exercise during concurrent training for rehabilitation does not alter acute genetic expression, mitochondrial enzyme activity or improvements in muscle function. PLoS One 9:e109189 (2014). WB ; Human . Read more (PubMed: 25289940) »
  • Chou JL  et al. Promoter hypermethylation of FBXO32, a novel TGF-beta/SMAD4 target gene and tumor suppressor, is associated with poor prognosis in human ovarian cancer. Lab Invest 90:414-25 (2010). IHC-P ; Human . Read more (PubMed: 20065949) »

See all 2 Publications for this product

Product Wall

Thank you for your reply. I have checked our catalog and we do have a positive control available, human skeletal muscle whole cell lysate ab29330. We do not have samples available, but if the antibodies produced many non-specific bands in WB, this i...

Read More

Thank you for contacting Abcam regarding out FBX32 and MURF1 antibodies. I would like to recommend two products that will be suitable for your experiments. Regarding Anti-Fbx32, I would like to recommend ab92281. As shown on the online datasheet, ...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skeletal muscle biopsy: Polymyositis)
Specification Skeletal muscle biopsy: Polymyositis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid HIER pH6 10mM
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jun 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"