Overview

  • Product nameAnti-Fbx32 antibody
    See all Fbx32 primary antibodies
  • Description
    Rabbit polyclonal to Fbx32
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Chicken, Cow, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Fbx32.

    (Peptide available as ab74022.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Skeletal Muscle, Mouse Skeletal Muscle, Rat Skeletal Muscle

Properties

Applications

Our Abpromise guarantee covers the use of ab74023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/500.
IHC-Fr 1/200.

Target

Anti-Fbx32 antibody images

  • All lanes : Anti-Fbx32 antibody (ab74023) at 2 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • ab74023 (1/2000) staining Fbx32 in paraffin-embedded Human heart tissue.  Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval.  The secondary antibody was goat anti rabbit conjugated to HRP.  For further experimental details please refer to abreview.

    See Abreview

  • ab74023 staining Fbx32 in rat muscle tissue sections by Immunohistochemistry (Frozen section). Tissue was fixed with 4% PFA overnight. Samples were then blocked with 10% donkey serum for 1 hour at 24°C followed by incubation with ab74023 at a 1/200 dilution, for 24 hours at 4°C. A pig anti-rabbit Alexa Fluoro 488 conjugate was used as secondary antibody at a 1/1000 dilution. Nuclei were stained using the standard Hoestch method and are depicted in blue.

    See Abreview

  • ab74023 staining cultured rat astrocytes by ICC/IF. The cultured astrocytes were fixed with paraformaldehyde and blocked with 10% donkey serum for 30 minutes at 24°C. The cultured astrocytes were then stained with ab74023 at 1/500 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4.5h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue.  FBX32 expressed in the newly born astrocytes but does not co-localise with mature astrocytes.

    See Abreview

References for Anti-Fbx32 antibody (ab74023)

This product has been referenced in:
  • Sun B  et al. The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia. Mol Med Rep 14:3113-21 (2016). Read more (PubMed: 27511050) »
  • Kley RA  et al. Pathophysiology of protein aggregation and extended phenotyping in filaminopathy. Brain 135:2642-60 (2012). Read more (PubMed: 22961544) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (8% gel)
Sample Pig Cell lysate - whole cell (smooth muscle cell)
Specification smooth muscle cell
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Sep 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (SD rat skeleta muscle tissue)
Loading amount 30 µg
Specification SD rat skeleta muscle tissue
Treatment 30mg/Kg STZ IP injection
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 16 2013

Thank you for your reply.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab32845.
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Thank you for contacting us.
I am sorry to hear the antibody is not performing as expected.

Please let me know if ab92281 would be a suitable replacement antibody, and I will arrange the shipment. Alternatively, a credit or a refund can ...

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Thank you for your enquiry.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Fbx32 antibody tested in the ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Astrocytes culture)
Specification Astrocytes culture
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 10 2012

Thank you for contacting us. The antibody ab74023 will be useful for IHC, but we do not know if it has equal affinity for free Fbx32 and the same protein bound to substrate or ubiquitin. The antibody is raised against a peptide from the C-terminus o...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Muscle)
Specification Muscle
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Heart)
Specification Heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted May 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"