Overview

  • Product nameAnti-Fbx32 antibody
    See all Fbx32 primary antibodies
  • Description
    Goat polyclonal to Fbx32
  • SpecificityExpected to recognize reported isoform 1 (NP_478136.1).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Synthetic peptide:

    C-NSKTKTQYFHQEK

    , corresponding to internal amino acids 70-82 of Human Fbx32 (NP_478136.1).(Peptide available as ab131394.)

  • Positive control
    • Human skeletal muscle lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa).Can be blocked with Fbx32 peptide (ab131394).

Target

Anti-Fbx32 antibody images

  • Anti-Fbx32 antibody (ab92281) at 0.1 µg/ml + Human skeletal muscle lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-Fbx32 antibody (ab92281)

This product has been referenced in:
  • Vendelbo MH  et al. Fasting increases human skeletal muscle net phenylalanine release and this is associated with decreased mTOR signaling. PLoS One 9:e102031 (2014). WB ; Human . Read more (PubMed: 25020061) »
  • Borgenvik M  et al. Intake of branched-chain amino acids influences the levels of MAFbx mRNA and MuRF-1 total protein in resting and exercising human muscle. Am J Physiol Endocrinol Metab 302:E510-21 (2012). WB ; Human . Read more (PubMed: 22127230) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.
We can provide the blocking peptide. The catalogue number is ab131394. I apologize for being late in getting back to you.
I hope this information is helpful to you. Please do not hesitate to contact us if you need...

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Thank you for contacting us.
I can confirm that we can provide the blocking peptide. I have contacted the datasheet team for publishing it online. I will tell you the catalogue number soon.
I hope this information is helpful to you. Please do...

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Thank you for your email.

As I was preparing the free of charge replacement for you, the system alerted me that ab92281 is not allowed to be shipped into Canada, due to Canadian Import restrictions which make this product unavailable to Cana...

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Thank you for your enquiry.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Fbx32 antibody tested in the ...

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Thank you for your enquiry and your interest in our products.



I can confirm that ab92281 is Anti-Fbx32 antibody and ab7132 is Rabbit anti-Goat IgG H&L (HRP) secondary antibody; and both products are still published and available. <...

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Thank you for your enquiry. I can recommend the following HRP-conjugated secondary antibody based on your requirements: Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed (ab7132) I hope this is helpful. Ple...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"