Recombinant
RabMAb

Anti-Fbx32 antibody [EPR9148(2)] (ab168372)

Overview

  • Product name
    Anti-Fbx32 antibody [EPR9148(2)]
    See all Fbx32 primary antibodies
  • Description
    Rabbit monoclonal [EPR9148(2)] to Fbx32
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Fbx32.
    Database link: Q969P5

  • Positive control
    • Human skeletal muscle, mouse heart, mouse muscle and rat muscle lysates; A673 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF 1/200.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
    • Tissue specificity
      Specifically expressed in cardiac and skeletal muscle.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Contains 1 F-box domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4833442G10Rik antibody
      • AI430017 antibody
      • Atrogin 1 antibody
      • Atrogin-1 antibody
      • ATROGIN1 antibody
      • Atrophy gene 1 antibody
      • F box only protein 32 antibody
      • F-box only protein 32 antibody
      • F-box protein 32 antibody
      • FBX32_HUMAN antibody
      • fbxo25 antibody
      • FBXO32 antibody
      • FLJ32424 antibody
      • MAFbx antibody
      • MGC108443 antibody
      • MGC137646 antibody
      • MGC33610 antibody
      • Muscle atrophy F box antibody
      • Muscle atrophy F box protein antibody
      • Muscle atrophy F-box protein antibody
      see all

    Images

    • All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified)

      Lane 1 : Mouse muscle
      Lane 2 : Rat muscle

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescent staining of A673 cells (fixed in 4% PFA) using purified ab168372 at a dilution of 1/200. An Alexa Fluor® 555 goat anti-rabbit (ab150082) was used at a dilution of 1/500 and the cells were counter-stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, purified ab168372 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/400.

    • Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified) + Human skeletal muscle at 10 µg

      Secondary
      HRP anti-rabbit IgG (specific to non-reduced IgG) at 1/1000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescent analysis of A673 cells labeling Fbx32 with ab168372 at 1/250 dilution.

    • All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (unpurified)

      Lane 1 : mouse heart lysate
      Lane 2 : rat muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 42 kDa

    References

    This product has been referenced in:
    • Jheng JR  et al. The double-edged sword of endoplasmic reticulum stress in uremic sarcopenia through myogenesis perturbation. J Cachexia Sarcopenia Muscle N/A:N/A (2018). Read more (PubMed: 29380555) »
    • Chen R  et al. Necrostatin-1 protects C2C12 myotubes from CoCl2-induced hypoxia. Int J Mol Med 41:2565-2572 (2018). Read more (PubMed: 29436688) »

    See all 11 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Rat Tissue lysate - other (muscle)
    Gel Running Conditions
    Reduced Denaturing (9)
    Loading amount
    30 µg
    Specification
    muscle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Mr. Baptiste Jude

    Verified customer

    Submitted Oct 10 2016

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8)
    Sample
    Mouse Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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