Overview

  • Product nameAnti-FBXL18 antibody
    See all FBXL18 primary antibodies
  • Description
    Mouse monoclonal to FBXL18
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-111 of Human FBXL18

Properties

Applications

Our Abpromise guarantee covers the use of ab57768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 88 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceFBXL18 is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
  • Database links
  • Alternative names
    • F box and leucine rich repeat protein 18 antibody
    • F box/LRR repeat protein 18 antibody
    • FBL18 antibody
    • FBXL 18 antibody
    • FLJ10776 antibody
    • FLJ11467 antibody
    • FLJ26934 antibody
    • FLJ38075 antibody
    • FLJ41541 antibody
    see all

Anti-FBXL18 antibody images

  • Overlay histogram showing A431 cells stained with ab57768 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57768, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • FBXL18 antibody (ab57768) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.


  • Predicted band size : 88 kDa
    FBXL18 antibody (ab57768) at 1ug/lane + A-431 cell lysate at 25ug/lane.

References for Anti-FBXL18 antibody (ab57768)

ab57768 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"