The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human FBXL2 peptide (ab23301).
FunctionCalcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin (By similarity). Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication.
Tissue specificityExpressed in brain, heart, kidney, liver, lung, pancreas and placenta.
ab17018 (10µg/ml) staining of FBXL2 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows cytoplasm staining in neuronal bodies.
Western blot - FBXL2 antibody (ab17018)
Predicted band size : 49 kDa ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence. ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence.
References for Anti-FBXL2 antibody (ab17018)
has not yet been referenced specifically in any publications.
Publishing research using ab17018? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"