Overview

  • Product name
  • Description
    Goat polyclonal to FBXL3
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide:

    KRGGRDSDRNSSEE-C

    , corresponding to N terminal amino acids 2-15 of Human FBXL3.

  • Positive control
    • Human thyroid tissue; Human adipose lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab115515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 49 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex involved in circadian clock function. The SCF(FBXL3) complex acts by mediating ubiquitination and subsequent degradation of CRY1 and CRY2. Recruiter of target protein that may recognize and bind to some phosphorylated proteins and promotes their ubiquitination and degradation.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 1 F-box domain.
    Contains 7 LRR (leucine-rich) repeats.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • F box and leucine rich repeat protein 3 antibody
    • F box and leucine rich repeat protein 3A antibody
    • F box protein Fbl3a antibody
    • F-box and leucine-rich repeat protein 3A antibody
    • F-box/LRR-repeat protein 3 antibody
    • F-box/LRR-repeat protein 3A antibody
    • FBL3 antibody
    • FBL3A antibody
    • Fbxl3 antibody
    • FBXL3_HUMAN antibody
    • FBXL3A antibody
    see all

Images

  • ab115515, at 5µg/ml staining FBXL3 in formalin-fixed, paraffin-embedded Human thyroid tissue by Immunohistochemistry, using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-FBXL3 antibody (ab115515) at 1 µg/ml + Human adipose lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 49 kDa

References

ab115515 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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