Overview

  • Product nameAnti-FBXL3 antibody
    See all FBXL3 primary antibodies
  • Description
    Mouse monoclonal to FBXL3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MKRGGRDSDR NSSEEGTAEK SKKLRTTNEH SQTCDWGNLL QDIILQVFKY LPLLDRAHAS QVCRNWNQVF HMPDLWRCFE FELNQPATSY LKATHPELIK , corresponding to amino acids 1-101 of Human FBXL3 (NP_036290) with a 26kDa enzyme tag

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

Target

  • FunctionSubstrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex involved in circadian clock function. The SCF(FBXL3) complex acts by mediating ubiquitination and subsequent degradation of CRY1 and CRY2. Recruiter of target protein that may recognize and bind to some phosphorylated proteins and promotes their ubiquitination and degradation.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 1 F-box domain.
    Contains 7 LRR (leucine-rich) repeats.
  • Cellular localizationNucleus. Cytoplasm. Predominantly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • F box and leucine rich repeat protein 3 antibody
    • F box and leucine rich repeat protein 3A antibody
    • F box protein Fbl3a antibody
    • F-box and leucine-rich repeat protein 3A antibody
    • F-box/LRR-repeat protein 3 antibody
    • F-box/LRR-repeat protein 3A antibody
    • FBL3 antibody
    • FBL3A antibody
    • Fbxl3 antibody
    • FBXL3_HUMAN antibody
    • FBXL3A antibody
    see all

Anti-FBXL3 antibody images

  • Anti-FBXL3 antibody (ab77200) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • Anti-FBXL3 antibody (ab77200) at 1/500 dilution + immunogen (37.11 KDa) at 0.1 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 49 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-FBXL3 antibody (ab77200)

ab77200 has not yet been referenced specifically in any publications.

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