Overview

  • Product name
  • Description
    Rabbit polyclonal to FbxL4
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 43-351 of Human FbxL4.

  • Positive control
    • A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cells; BT474 xenograft.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-FbxL4 antibody (ab153812) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size : 70 kDa
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling FbxL4 with ab153812 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded BT474 xenograft labeling FbxL4 with ab153812 at 1/100 dilution.

References

ab153812 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

Based on sequence homology ab153812 does not recognize other FBL protein family.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your response.

Based on sequence homology ab153812 does not recognize other FBL protein family.

If you need any further assistance in the future, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up