Overview

  • Product nameAnti-FBXL5 antibody
    See all FBXL5 primary antibodies
  • Description
    Rabbit polyclonal to FBXL5
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to N terminal amino acids 93-123 of Human FBXL5(Peptide available as ab107024.)

  • Positive control
    • WB: HepG2 and Mouse heart tissue lysates IHC-P: Testis tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab102692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 79 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat-mediated/Sodium citrate pH 6).

Target

  • FunctionComponent of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 F-box domain.
    Contains 7 LRR (leucine-rich) repeats.
  • DomainThe hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded.
  • Post-translational
    modifications
    Ubiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • F box and leucine rich repeat protein 5 antibody
    • F box protein FBL5 antibody
    • F box/LRR repeat protein 5 antibody
    • F-box and leucine-rich repeat protein 5 antibody
    • F-box protein FBL4/FBL5 antibody
    • F-box/LRR-repeat protein 5 antibody
    • FBL4 antibody
    • FBL5 antibody
    • FBXL5 antibody
    • FBXL5_HUMAN antibody
    • FLR1 antibody
    • p45SKP2 like protein antibody
    • p45SKP2-like protein antibody
    see all

Anti-FBXL5 antibody images

  • Anti-FBXL5 antibody (ab102692) at 1/100 dilution + HepG2 cell line lysates at 35 µg

    Predicted band size : 79 kDa
  • Anti-FBXL5 antibody (ab102692) at 1/100 dilution + Mouse heart tissue lysates at 35 µg

    Predicted band size : 79 kDa
  • Immunohistochemical analysis of FBXL5 in formalin fixed, and paraffin embedded testis tissue using 1/50 ab102693 followed by peroxidase conjugation of the secondary antibody and DAB staining.

References for Anti-FBXL5 antibody (ab102692)

This product has been referenced in:
  • Sammarco MC  et al. Endogenous bone regeneration is dependent upon a dynamic oxygen event. J Bone Miner Res 29:2336-45 (2014). IHC ; Mouse . Read more (PubMed: 24753124) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
Sample Human Cell (MCF7)
Specification MCF7
Permeabilization Yes - Triton X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 26 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mice embryo 18dpc)
Specification Mice embryo 18dpc
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
Permeabilization No
Blocking step 1% BSA 1%FBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Raul Peña

Verified customer

Submitted Dec 18 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Colorectal Carcinoma)
Specification Human Colorectal Carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step 1% FBS 1%BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"