Overview

  • Product nameAnti-FBXL5 antibody
    See all FBXL5 primary antibodies
  • Description
    Mouse polyclonal to FBXL5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-691 of Human FBXL5

  • Positive control
    • FBXL5 transfected mammalian cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 79 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesContains 1 F-box domain.
      Contains 7 LRR (leucine-rich) repeats.
    • DomainThe hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded.
    • Post-translational
      modifications
      Ubiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor.
    • Cellular localizationCytoplasm > perinuclear region.
    • Information by UniProt
    • Database links
    • Alternative names
      • F box and leucine rich repeat protein 5 antibody
      • F box protein FBL5 antibody
      • F box/LRR repeat protein 5 antibody
      • F-box and leucine-rich repeat protein 5 antibody
      • F-box protein FBL4/FBL5 antibody
      • F-box/LRR-repeat protein 5 antibody
      • FBL4 antibody
      • FBL5 antibody
      • FBXL5 antibody
      • FBXL5_HUMAN antibody
      • FLR1 antibody
      • p45SKP2 like protein antibody
      • p45SKP2-like protein antibody
      see all

    Anti-FBXL5 antibody images

    • All lanes : Anti-FBXL5 antibody (ab68069) at 1/500 dilution

      Lane 1 : FBXL5 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 79 kDa
      Observed band size : 85 kDa (why is the actual band size different from the predicted?)

    References for Anti-FBXL5 antibody (ab68069)

    This product has been referenced in:
    • Chen ZW  et al. FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic Acids Res 42:11560-9 (2014). Read more (PubMed: 25249620) »

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample Mouse Tissue sections (Mouse 14.5dpc)
    Specification Mouse 14.5dpc
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 14 2013

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample Human Cell (MCF7)
    Specification MCF7
    Permeabilization Yes - 0,5 Tw-20 in PBS
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"