Overview

  • Product name
    Anti-FbxL7 antibody
  • Description
    Rabbit polyclonal to FbxL7
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • Immunogen

    Synthetic peptide: KEQASIDRLP DHSMVQIFSF LPTNQLCRCA RVCRRWYNLA WDPRLWRTIR, corresponding to amino acids 109-158 of Human FbxL7

  • Positive control
    • HepG2 cell lysate and human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay:1/312500.

Target

  • Function
    Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
  • Sequence similarities
    Contains 1 F-box domain.
    Contains 11 LRR (leucine-rich) repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL023057 antibody
    • D230018M15Rik antibody
    • F Box and Leucine Rich Repeat Protein 7 antibody
    • F box protein FBL6/FBL7 antibody
    • F box/LRR-repeat protein 7 antibody
    • F-box and leucine-rich repeat protein 7 antibody
    • F-box protein FBL6/FBL7 antibody
    • F-box/LRR-repeat protein 7 antibody
    • FBL 6 antibody
    • FBL 7 antibody
    • FBL6 antibody
    • FBL7 antibody
    • FBXL 7 antibody
    • FBXL7 antibody
    • FBXL7_HUMAN antibody
    • FLJ11609 antibody
    • KIAA0840 antibody
    • MGC102204 antibody
    see all

Images

  • Anti-FbxL7 antibody (ab59149) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FbxL7 with ab59149 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References

ab59149 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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