Overview

  • Product nameAnti-FBXO21 antibody
    See all FBXO21 primary antibodies
  • Description
    Rabbit polyclonal to FBXO21
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Marmoset (common)
  • Immunogen

    Synthetic peptide: VWKEQFRVRW PSLMKHYSPT DYVNWLEEYK VRQKAGLEAR KIVASFSKRF , corresponding to N terminal amino acids 72-121 of Human FBXO21

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-FBXO21 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling FBXO21 with ab80433 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the membrane and cytoplasm of alveolar type I cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - FBXO21. Right - Merge.
  • Anti-FBXO21 antibody (ab80433) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 71 kDa
    Observed band size : 71 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References for Anti-FBXO21 antibody (ab80433)

ab80433 has not yet been referenced specifically in any publications.

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