Overview

  • Product nameAnti-FBXO22 antibody
    See all FBXO22 primary antibodies
  • Description
    Rabbit polyclonal to FBXO22
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 227-276 (CCKVGASNYL QQVVSTFSDM NIILAGGQVD NLSSLTSEKY VLCASDFVCE) of Human FBXO22 (NP_036302).

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.

Target

Anti-FBXO22 antibody images

  • Anti-FBXO22 antibody (ab82974) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-FBXO22 antibody (ab82974)

ab82974 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"